Evaluation of biological debris on reusable endodontic instruments subjected to different cleaning methods prior to sterilization

  • Poorva Khullar Post-Graduate second year, Department of Conservative Dentistry and Endodontics, Mahatma Gandhi Dental College and Hospital, Jaipur, India
  • Deepak Raisingani Mahatma Gandhi Dental College and Hospital,Mahatma Gandhi University of Medical Sciences and Technology Sitapura, Jaipur, 302022, INDIA
  • Shailendra Gupta Mahatma Gandhi Dental College and Hospital,,Mahatma Gandhi University of Medical Sciences and Technology Sitapura, Jaipur, 302022, INDIA
  • Kundendu A Bishen Mahatma Gandhi Dental College and Hospital,,Mahatma Gandhi University of Medical Sciences and Technology Sitapura, Jaipur, 302022, INDIA

Abstract

The aim of this study was to evaluate the presence of biological debris on reusable endodontic instruments subjected to different cleaning methods prior to sterilization. Sixty endodontic hand instruments (K-files #15-40 used in 3-4 teeth for cleaning and shaping) were analysed and were divided into 3 groups on the basis of decontamination protocols used. Twenty new K-files were used as controls. The evaluation of the effectiveness of the cleaning methods used was based on the amount of residual debris detected by immersion of instruments in Van Gieson’s solution for 3 minutes. The samples were then rinsed in distilled water and dried on endodontic stand and analyzed by light microscopy. Residual biological debris was observed on 93% of all the samples taken. The mean value of Maximum Biologic Contamination (MBC) was 20% for the group where instruments were brushed manually and immersed in alcohol, 15% in the group in which commercially available disinfectant were used and 11% for the group where instruments were ultrasonically cleaned. There was statistically significant difference between the cleaning protocols applied (P< 0.001). The methods used to clean endodontic instruments appear to be generally ineffective for the removal of biological debris. The best method was the one that included mechanical, chemical and ultrasonic cleaning of the instruments.

Author Biographies

Poorva Khullar, Post-Graduate second year, Department of Conservative Dentistry and Endodontics, Mahatma Gandhi Dental College and Hospital, Jaipur, India
Post-Graduate second year, Department of Conservative Dentistry and Endodontics, Mahatma Gandhi Dental College and Hospital, Jaipur, India
Deepak Raisingani, Mahatma Gandhi Dental College and Hospital,Mahatma Gandhi University of Medical Sciences and Technology Sitapura, Jaipur, 302022, INDIA
MDS, Professor, Department of Conservative Dentistry and Endodontics
Shailendra Gupta, Mahatma Gandhi Dental College and Hospital,,Mahatma Gandhi University of Medical Sciences and Technology Sitapura, Jaipur, 302022, INDIA
MDS, Professor and HOD, Department of Conservative Dentistry and Endodontics
Kundendu A Bishen, Mahatma Gandhi Dental College and Hospital,,Mahatma Gandhi University of Medical Sciences and Technology Sitapura, Jaipur, 302022, INDIA
MDS(Reader) Department of Oral and Maxillofacial Pathology
Published
2013-05-20
Section
Original Articles